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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
11/09/2014 |
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Actualizado : |
30/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Autor : |
BOVE, R.; LÓPEZ, F.; PERERA, C.; CARRACELAS, B.; TORRES, D.; DE SOUZA, G.; AZAMBUJA, C.; BERMÚDEZ, J.; ALZUGARAY, F.; MEDEROS, A. |
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Afiliación : |
DILAVE.; DILAVE.; DILAVE.; EMERITA BEATRIZ CARRACELAS MARQUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO GABRIEL TORRES DINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO DE SOUZA CAMARGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Laboratorio Genia, Montevideo, Uruguay.; Facultad de Veterinaria, UdelaR, Uruguay.; Facultad de Veterinaria, UdelaR, Uruguay.; AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Diagnóstico Campylobacter fetus venerealis por PCR, en un aborto bovino espontáneo // Diagnosis of Campylobacter fetus venerealis in aborted bovine fetus. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Veterinaria (Montevideo), 2013, v. 49, no. 192, p. 20-28. |
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Idioma : |
Español |
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Notas : |
History article: Recibido: 25/02/2013; Aprobado: 01/04/2013. |
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Contenido : |
En este trabajo se reporta el diagnóstico de Campylobacter fetus venerealis (Cfv) de un aborto bovino espontáneo utilizando la técnica de reacción en cadena de la polimerasa (PCR) múltiple. La muestra de fluido estomacal fetal fue sometida a otros métodos de diagnóstico para Campylobacter fetus: cultivo bacteriano e inmunoflurescencia directa (IFD). Adicionalmente se emplearon marcadores moleculares específicos de CFv que permitieron identificar una banda de 142pb asociada al gen plasmídico parA Cfv. Dicho amplicón fue secuenciado y analizado por blastN. Los resultados de los cultivos bacteriológicos e IFD evidenciaron la presencia de Campylobacter fetus y los resultados moleculares identificaron la subespecie venerealis. El resultado de la secuenciación reveló un e-value de 3e-33 con un valor de identidad del 99%, confirmando la subespecie venerealis // The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-value with a 99% identity, confirming subspecies venerealis. MenosEn este trabajo se reporta el diagnóstico de Campylobacter fetus venerealis (Cfv) de un aborto bovino espontáneo utilizando la técnica de reacción en cadena de la polimerasa (PCR) múltiple. La muestra de fluido estomacal fetal fue sometida a otros métodos de diagnóstico para Campylobacter fetus: cultivo bacteriano e inmunoflurescencia directa (IFD). Adicionalmente se emplearon marcadores moleculares específicos de CFv que permitieron identificar una banda de 142pb asociada al gen plasmídico parA Cfv. Dicho amplicón fue secuenciado y analizado por blastN. Los resultados de los cultivos bacteriológicos e IFD evidenciaron la presencia de Campylobacter fetus y los resultados moleculares identificaron la subespecie venerealis. El resultado de la secuenciación reveló un e-value de 3e-33 con un valor de identidad del 99%, confirmando la subespecie venerealis // The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-... Presentar Todo |
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Palabras claves : |
ABORTION; ABORTO; BOVINE; BOVINOS; CAMPYLOBACTER FETUS VENEREALIS; PCR; REACCIÓN EN CADENA DE LA POLIMERASA (PCR) MÚLTIPLE. |
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Thesagro : |
CAMPYLOBACTER. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3087/1/CDocuments-and-SettingsachiacchioMis-documentosA-BIBLIOTECA-INIA-TACUAREMBO-TODOARTICULOS-TECNICOS-INIA-EN-REVISTAS-ARBITRADASINIA-TACUAREMBOCARNE-Y-LANAVeterinaria-Montevideo2013v49n192p20-28.pdf
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Marc : |
LEADER 02600naa a2200337 a 4500
001 1050145
005 2019-09-30
008 2013 bl uuuu u00u1 u #d
100 1 $aBOVE, R.
245 $aDiagnóstico Campylobacter fetus venerealis por PCR, en un aborto bovino espontáneo // Diagnosis of Campylobacter fetus venerealis in aborted bovine fetus.
260 $c2013
500 $aHistory article: Recibido: 25/02/2013; Aprobado: 01/04/2013.
520 $aEn este trabajo se reporta el diagnóstico de Campylobacter fetus venerealis (Cfv) de un aborto bovino espontáneo utilizando la técnica de reacción en cadena de la polimerasa (PCR) múltiple. La muestra de fluido estomacal fetal fue sometida a otros métodos de diagnóstico para Campylobacter fetus: cultivo bacteriano e inmunoflurescencia directa (IFD). Adicionalmente se emplearon marcadores moleculares específicos de CFv que permitieron identificar una banda de 142pb asociada al gen plasmídico parA Cfv. Dicho amplicón fue secuenciado y analizado por blastN. Los resultados de los cultivos bacteriológicos e IFD evidenciaron la presencia de Campylobacter fetus y los resultados moleculares identificaron la subespecie venerealis. El resultado de la secuenciación reveló un e-value de 3e-33 con un valor de identidad del 99%, confirmando la subespecie venerealis // The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-value with a 99% identity, confirming subspecies venerealis.
650 $aCAMPYLOBACTER
653 $aABORTION
653 $aABORTO
653 $aBOVINE
653 $aBOVINOS
653 $aCAMPYLOBACTER FETUS VENEREALIS
653 $aPCR
653 $aREACCIÓN EN CADENA DE LA POLIMERASA (PCR) MÚLTIPLE
700 1 $aLÓPEZ, F.
700 1 $aPERERA, C.
700 1 $aCARRACELAS, B.
700 1 $aTORRES, D.
700 1 $aDE SOUZA, G.
700 1 $aAZAMBUJA, C.
700 1 $aBERMÚDEZ, J.
700 1 $aALZUGARAY, F.
700 1 $aMEDEROS, A.
773 $tVeterinaria (Montevideo), 2013$gv. 49, no. 192, p. 20-28.
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INIA Tacuarembó (TBO) |
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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
08/08/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
ANDRIGHETTO CANOZZIA, M.E.; MEDEROS, A.; MANTECA, X.; TURNER, S.; MCMANUS, C.; ZAGO, D.; JARDIM BARCELLOS, J.O. |
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Afiliación : |
MARÍA EUGENIA ANDRIGHETTO CANOZZIA, Department of Animal Science, Federal University of Rio Grande do Sul.; AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; XAVIER MANTECA, Department of Animal and Food Science, School of Veterinary Science, Universitat Autònoma de Barcelona.; SIMON TURNER, Animal and Veterinary Sciences Group, Scotland's Rural College.; CONCEPTA MCMANUS, Institute of Biological Sciences, University of Brasilia.; DANIELE ZAGO, Department of Animal Science, Federal University of Rio Grande do Sul.; JÚLIO OTÁVIO JARDIM BARCELLOS, Department of Animal Science, Federal University of Rio Grande do Sul. |
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Título : |
A meta-analysis of cortisol concentration, vocalization, and average daily gain associated with castration in beef cattle. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Research in Veterinary Science, 2017, no. 114, p. 430-443. |
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DOI : |
10.1016/j.rvsc.2017.07.014 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 21 December 2016 // Received in revised form: 4 July 2017 // Accepted 16 July 2017 |
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Contenido : |
A systematic review and meta-analysis (MA) were performed to summarize all scientific evidence for the effects of castration in male beef cattle on welfare indicators based on cortisol concentration, average daily gain (ADG), and vocalization. We searched five electronic databases, conference proceedings, and experts were contacted
electronically. The main inclusion criteria involved completed studies using beef cattle up to one year of age undergoing surgical and non-surgical castration that presented cortisol concentration, ADG, or vocalization as an outcome. A random effect MA was conducted for each indicator separately with the mean of the control and
treated groups. A total of 20 publications reporting 26 studies and 162 trials were included in the MA involving 1814 cattle. Between study heterogeneity was observed when analysing cortisol (I2 =56.7%) and ADG (I2 =79.6%). Surgical and non-surgical castration without drug administration compared to uncastrated animals
showed no change (P?0.05) in cortisol level. Multimodal therapy for pain did not decrease (P ?0.05) cortisol concentration after 30 min when non-surgical castration was performed. Comparison between surgical castration, with and without anaesthesia, showed a tendency (P=0.077) to decrease cortisol levels after 120 min of intervention. Non-surgical and surgical castration, performed with no pain mitigation, increased and tended to increase the ADG by 0.814 g/d (P=0.001) and by 0.140 g/d (P=0.091), respectively, when compared to a non-castrated group. Our MA study demonstrates an inconclusive result to draw recommendations on preferred castration practices to minimize pain in beef cattle. MenosA systematic review and meta-analysis (MA) were performed to summarize all scientific evidence for the effects of castration in male beef cattle on welfare indicators based on cortisol concentration, average daily gain (ADG), and vocalization. We searched five electronic databases, conference proceedings, and experts were contacted
electronically. The main inclusion criteria involved completed studies using beef cattle up to one year of age undergoing surgical and non-surgical castration that presented cortisol concentration, ADG, or vocalization as an outcome. A random effect MA was conducted for each indicator separately with the mean of the control and
treated groups. A total of 20 publications reporting 26 studies and 162 trials were included in the MA involving 1814 cattle. Between study heterogeneity was observed when analysing cortisol (I2 =56.7%) and ADG (I2 =79.6%). Surgical and non-surgical castration without drug administration compared to uncastrated animals
showed no change (P?0.05) in cortisol level. Multimodal therapy for pain did not decrease (P ?0.05) cortisol concentration after 30 min when non-surgical castration was performed. Comparison between surgical castration, with and without anaesthesia, showed a tendency (P=0.077) to decrease cortisol levels after 120 min of intervention. Non-surgical and surgical castration, performed with no pain mitigation, increased and tended to increase the ADG by 0.814 g/d (P=0.001) and by 0.140 g/d (P=0.091), respectively... Presentar Todo |
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Palabras claves : |
ANALGESIA; ANIMAL WELFARE; PAIN. |
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Thesagro : |
BIENESTAR ANIMAL; CASTRACION; CIENCIAS VETERINARIAS; GANADO DE CARNE. |
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Asunto categoría : |
L70 Ciencias veterinarias e higiene - Aspectos generales |
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Marc : |
LEADER 02654naa a2200301 a 4500
001 1028593
005 2019-08-08
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.rvsc.2017.07.014$2DOI
100 1 $aANDRIGHETTO CANOZZIA, M.E.
245 $aA meta-analysis of cortisol concentration, vocalization, and average daily gain associated with castration in beef cattle.
260 $c2017
500 $aArticle history: Received: 21 December 2016 // Received in revised form: 4 July 2017 // Accepted 16 July 2017
520 $aA systematic review and meta-analysis (MA) were performed to summarize all scientific evidence for the effects of castration in male beef cattle on welfare indicators based on cortisol concentration, average daily gain (ADG), and vocalization. We searched five electronic databases, conference proceedings, and experts were contacted electronically. The main inclusion criteria involved completed studies using beef cattle up to one year of age undergoing surgical and non-surgical castration that presented cortisol concentration, ADG, or vocalization as an outcome. A random effect MA was conducted for each indicator separately with the mean of the control and treated groups. A total of 20 publications reporting 26 studies and 162 trials were included in the MA involving 1814 cattle. Between study heterogeneity was observed when analysing cortisol (I2 =56.7%) and ADG (I2 =79.6%). Surgical and non-surgical castration without drug administration compared to uncastrated animals showed no change (P?0.05) in cortisol level. Multimodal therapy for pain did not decrease (P ?0.05) cortisol concentration after 30 min when non-surgical castration was performed. Comparison between surgical castration, with and without anaesthesia, showed a tendency (P=0.077) to decrease cortisol levels after 120 min of intervention. Non-surgical and surgical castration, performed with no pain mitigation, increased and tended to increase the ADG by 0.814 g/d (P=0.001) and by 0.140 g/d (P=0.091), respectively, when compared to a non-castrated group. Our MA study demonstrates an inconclusive result to draw recommendations on preferred castration practices to minimize pain in beef cattle.
650 $aBIENESTAR ANIMAL
650 $aCASTRACION
650 $aCIENCIAS VETERINARIAS
650 $aGANADO DE CARNE
653 $aANALGESIA
653 $aANIMAL WELFARE
653 $aPAIN
700 1 $aMEDEROS, A.
700 1 $aMANTECA, X.
700 1 $aTURNER, S.
700 1 $aMCMANUS, C.
700 1 $aZAGO, D.
700 1 $aJARDIM BARCELLOS, J.O.
773 $tResearch in Veterinary Science, 2017, no. 114, p. 430-443.
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